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    3. Cell-Free Circulating DNA: Purification and Analysis Techniques

    Cell-Free Circulating DNA: Purification and Analysis Techniques

    Posted By: yoyoloit
    Date: 16 Dec 2023 23:58:41
    Cell-Free Circulating DNA: Purification and Analysis Techniques

    Cell-Free Circulating DNA: Purification and Analysis Techniques (296 Pages)
    by Kristina Warton & Goli Samimi
    English | 2022 | ISBN: 9811244677 | 297 pages | True PDF EPUB | 26.76 MB


    Circulating cell-free DNA is poised to transform cancer diagnosis and care; however, it carries technical challenges such as low abundance, fragmentation and sensitivity to biospecimen handling. Development of clinically useful assays hinges on understanding the unique technical aspects of working with cell-free DNA as a substrate. This book provides an in-depth summary of the technical issues impacting cell-free DNA purification from blood plasma and analysis in a cancer context, including design of PCR assays, sequencing library preparation and methylation analysis. Emerging fields such as extracellular vesicles and blood nucleases are also covered, as well as basic biology and considerations pertinent to biobanking.

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